Active Constituents from Liriope platyphylla Root against Cancer Growth In Vitro
Figure 4
Characterization of apoptotic and autophagic death induced by LPRP-9. (a) Immunoblots results showing indicated doses of LPRP-9- or doxorubicin-induced apoptosis by detecting levels of procaspase 8, procaspase 9, and PARP cleavage for MCF-7 (left) and Huh-7 (right), and procaspase 7 or procaspase 3 for MCF-7 or Huh-7 cells, respectively. (b) Chromosomal fragmentation detection results for MCF-7 (left) and Huh-7 (right) showing chromosome integrity change due to LPRP-9 or doxorubicin treatment. (c) Immunoblots result of autophagy marker LC3 conversion was detected in MCF-7 (left) and Huh-7 (right). (d) Effects of Z-VAD-FMK and 3-MA on doxorubicin, resveratrol or LPRP-9-induced cell death by detecting cell viability using MTT assay at 24 h of drugs incubation. . * compared with null control within group. # compared with the Z-VAD-FMK treatment within group.
