The protective effects of BV on cisplatin-induced nephrotoxicity in mice. Mice received BV (1 mg/kg) once a day for 5 days. The control group received the same volume of PBS. After the fifth administration of BV or PBS, all mice received a single injection of cisplatin (25 mg/kg). Blood samples were obtained at 24 h and 48 h after cisplatin administration, and mice were sacrificed under ether anesthesia at 72 h after cisplatin administration. Blood and kidney samples were extracted for analysis. Blood was obtained at 24, 48, and 72 h after cisplatin injection (). Renal dysfunction was measured as creatinine (a) and BUN (b). Kidney sections were stained with H&E 72 h after cisplatin injection. PBS treatment alone (cont); PBS and cisplatin treatments (Cis); BV and cisplatin (Cis + BV) (400x, bar = 50 um) (c). Tubular injury damage was scored from 0 to 4 (0, none; 1, <10%; 2, 10–25%; 3, 25–75%; 4, >75%) (d). The values shown indicate the mean ± SEM. NS: no significance (). , versus Cis.