Figure 6: Effect of PC-SPESII on MDA-MB-231 cell migration. (a) Images of wound healing assays (100x magnification). Cells were seeded into 12-well cell culture plates, cultured in DMEM supplemented with 10% FBS, and allowed to grow to near confluence. Confluent monolayers were carefully wounded and the cellular debris was gently washed away with PBS. The wounded monolayer was reincubated in FBS-free DMEM containing 0, 0.25, 0.5, or 1 μL/mL of PC-SPESII for 24 or 48 hours. (b) Transwell chamber was performed for the migration assay (200x magnification). MDA-MB-231 cells were treated with 0 (B), 0.25 (C), 0.5 (D), or 1 (E) μL/mL of PC-SPESII for 24 hours during assay. No cells were seeded in (A). (c) Stand and error bars represent three independent experiments and each experiment was done in triplicate (* and ** compared to untreated control).