Flavonoids Identified from Korean Scutellaria baicalensis Georgi Inhibit Inflammatory Signaling by Suppressing Activation of NF-κB and MAPK in RAW 264.7 Cells
Figure 3
The inhibitory effect of flavonoids on expression of proinflammatory mediators such as cyclooxygenase-2 (COX-2) protein (a), COX-2 mRNA (a-1), inducible nitric oxide synthase (iNOS) protein (a-2), and iNOS mRNA (a-3), and (b) the effect of Scutellaria baicalensis flavonoids on NF-κB signal pathway was determined by Western blot assay. The cells were pretreated for 1 h with the indicated concentrations of flavonoids, followed by stimulation with LPS (1 μg/mL) for 24 h and 6 h in protein and mRNA, respectively. In NF- κB signal pathway analysis, RAW 264.7 cells were pretreated for 1 h with the indicated concentrations of flavonoids, followed by stimulation with LPS (1 μg/mL) for 30 min. Data shown are the mean standard deviation. Three independent experiments and differences between mean values were assessed by Student’s t-test. (*) indicates an increased pattern relative to the control (), and (**) indicates a decreased pattern relative to the lipopolysaccharide (LPS) group ().
