Simultaneous Determination of Crypto-Chlorogenic Acid, Isoquercetin, and Astragalin Contents in Moringa oleifera Leaf Extracts by TLC-Densitometric Method

Table 1

Method validation parameters for the quantitation of crypto-chlorogenic acid, isoquercetin, and astragalin by the proposed TLC-densitometric method.

Parameter	Results
	Crypto-chlorogenic acid	Isoquercetin	Astragalin
Linear range (ng/spot)	103–505	105–510	100–500
Calibration equation	𝑦=−2589.922+15.123𝑥	𝑦=−884.153+16.772𝑥	𝑦=511.081+20.486𝑥
Correlation coefficient (𝑟)	0.9961	0.9975	0.9968
LOQ (ng)	48.93	45.99	36.13
LOD (ng)	14.68	13.80	10.84

𝑥 is the amount of each standard in ng and 𝑦 is the peak area at 340 nm.