Simultaneous Determination of Crypto-Chlorogenic Acid, Isoquercetin, and Astragalin Contents in <i>Moringa oleifera</i> Leaf Extracts by TLC-Densitometric Method

<table class="table-group" id="tab4"><tr><td><table class="table"><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr class="thead"><td align="left">Parameter</td><td align="center" colspan="3">RSD (%)*</td></tr><tr class="thead"><td align="left"> </td><td align="center">Crypto-chlorogenic acid</td><td align="center">Isoquercetin</td><td align="center">Astragalin</td></tr><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr><td align="left">Mobile phase composition ratio</td><td align="center">2.99</td><td align="center">2.15</td><td align="center">1.20</td></tr><tr><td align="left">Time from spotting to chromatography</td><td align="center">1.59</td><td align="center">1.65</td><td align="center">0.97</td></tr><tr><td align="left">Time from chromatography to scanning</td><td align="center">1.16</td><td align="center">0.89</td><td align="center">0.79</td></tr><tr><td align="left">The length of the chromatogram</td><td align="center">1.81</td><td align="center">1.05</td><td align="center">1.21</td></tr><tr><td align="left">Presaturation period</td><td align="center">2.53</td><td align="center">0.66</td><td align="center">1.04</td></tr><tr class="table-tr"><td colspan="4"><hr class="tbody-hr"/></td></tr></table></td></tr><tr class="table-fn"><td><svg height="13.75" id="M34" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 7.7375002 13.75" width="7.7375002" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.012,-0,0,-.012,.062,5.525)"><path d="M471 153q-22 -15 -61 -13q-25 31 -45.5 49.5t-56.5 41.5q4 -71 28 -134q-17 -33 -42 -46q-24 12 -42 46q24 63 28 134q-36 -23 -56.5 -41.5t-45.5 -49.5q-36 -2 -61 13q0 28 19 59q65 10 130 43q-65 33 -130 43q-19 31 -19 59q22 15 61 13q25 -31 45.5 -49.5t56.5 -41.5
q-4 71 -28 134q17 33 42 46q24 -12 42 -46q-24 -63 -28 -134q36 23 56.5 41.5t45.5 49.5q36 2 61 -13q0 -28 -19 -59q-65 -10 -130 -43q65 -33 130 -43q19 -31 19 -59z" id="x2217"></path></g>
</svg>Value from six determinations.<br/></td></tr></table>

Robustness studies of crypto-chlorogenic acid, isoquercetin, and astragalin.

Evidence-Based Complementary and Alternative Medicine

tab4

Table 4

Table 4: Simultaneous Determination of Crypto-Chlorogenic Acid, Isoquercetin, and Astragalin Contents in <i>Moringa oleifera</i> Leaf Extracts by TLC-Densitometric Method 

Table 4 | Simultaneous Determination of Crypto-Chlorogenic Acid, Isoquercetin, and Astragalin Contents in Moringa oleifera Leaf Extracts by TLC-Densitometric Method 