Electroacupuncture Could Regulate the NF-𝜿B Signaling Pathway to Ameliorate the Inflammatory Injury in Focal Cerebral Ischemia/Reperfusion Model Rats

Table 1

Primer sequences and annealing temperatures employed for Q-PCR.

Primer name	Sequence	Annealing temperature
NF-κB p65	F 5′-ATCGTGGAGCACTTGGTGACT-3′; R 5′-GCCCTGGTAGGTTACTCTGTTGA-3′	55.2°C
IκBα	F 5′-GTTTCCCCTCATCTTTCCCTCA-3′; R 5′-GGGTGCGTCTTAGTGGTATCTGT-3′	56.7°C
IKKα	F 5′-GTCAGGAGAAGTTCGGTTTGA-3′; R 5′-ATTCCAGTTTCACGCTCATGGAT-3	54.2°C
IKKβ	F 5′-GTCTTGTTGATGGTTCCTGACT-3′; R 5′-AAGACAAACGAGGGCCTCACAT-3′	56.0°C
GAPDH	F 5′-CTTAGCACCCCTGGCCAAG-3′; R 5′-GATGTTCTGGAGAGCCCCG-3′	54.0°C