Anti-Proliferative Effect of an Aqueous Extract of Prunella vulgaris in Vascular Smooth Muscle Cells
Figure 4
(a) Effect of APV on high glucose-induced NF-kappaB activation. The HASMCs were transiently transfected with pGL3-4κB-Luc and pCMV-β-gal. This was followed by harvesting, and their luciferase activities were determined. Values were expressed as mean ± S.E. of three individual experiments. ** versus control; # versus high glucose alone. (b) Effect of APV on high glucose-induced MAPKs activation. The total cellular protein (40 μg) extracts were prepared and separated on 10% SDS-PAGE and blotted with the antibodies specific for p-ERK, p-p38, and p-JNK. Each electrophoretogram is representative of the results from three individual experiments.
