Anti-Proliferative Effect of an Aqueous Extract of Prunella vulgaris in Vascular Smooth Muscle Cells
Figure 5
(a) Effect of APV on high glucose-induced expression of Nrf2 and translocation into nucleus in HASMCs. Cytoplasm and nuclei fractions were extracted, and protein levels were determined by Western blot analysis. Values were expressed as mean ± S.E. of three individual experiments. (b) Effect of APV on high glucose-induced expression of HO-1. The total cellular protein (40 μg) extracts were prepared and separated on 10% SDS-PAGE and blotted with the antibody specific for HO-1 and β-actin. Each electrophoretogram is representative of the results from three individual experiments.