Acetylshikonin, a Novel AChE Inhibitor, Inhibits Apoptosis via Upregulation of Heme Oxygenase-1 Expression in SH-SY5Y Cells
Figure 1
Potential AChE inhibitors of natural products attenuated H2O2-induced cell death in both SH-SY5Y and PC12 cells. SH-SY5Y (a) or PC12 (b) cells were cultured with desired concentration of H2O2 for 4 hours; the cell viability was detected by MTT. Data shown are means ± SEM of results from independent experiments in triplicate. * compared with control cells. SH-SY5Y (c) or PC12 (d) cells were incubated with different potential AChE inhibitors (10 μM) for 12 hours and then stimulated with H2O2 (500 μM) for 4 hours, and the viability was detected by MTT. Data shown are means ± SEM of results from independent experiments in triplicate. * compared with control cells; # compared with H2O2-stimulated cells. For cytotoxicity test, SH-SY5Y (e) or PC12 (f) cells were incubated with different potential AChE inhibitors (10 μM) for 12 hours, and the viability was detected by MTT. Data shown are means ± SEM of results from independent experiments in triplicate. * compared with control cells.
