Essential Oil of Pinus koraiensis Exerts Antiobesic and Hypolipidemic Activity via Inhibition of Peroxisome Proliferator-Activated Receptors Gamma Signaling
Figure 2
Effects of EOPK on adipocyte differentiation of 3T3-L1 preadipocytes. 3T3-L1 preadipocytes were incubated in medium containing insulin (1.0 μg/mL) with or without the indicated concentrations of EOPK or GW9662. (a) Total RNA was extracted from 3T3-L1 (preadipocytes or adipocytes) cells treated with EOPK and used for RT-PCR analysis of PPARγ, C/EBPα, GPDH, and β-actin. (b) Total proteins prepared from EOPK-treated 3T3-L1 (preadipocytes or adipocytes) cells were subjected to western blot analysis of PPARγ, C/EBPα FABP, and β-actin. (c) Total proteins prepared from EOPK- or GW9662-treated 3T3-L1 (preadipocytes or adipocytes) cells were subjected to western blot analysis of PPARγ, FABP, and β-actin. (d) Cells were fixed and stained with Oil-Red O. The Oil-Red O-stained adipocytes were photographed at a ×100 magnification under a microscope. (e) Lipids were extracted using isopropanol, and the Oil-Red O was then analyzed at a wavelength of 520 nm. Values are presented as means ± SE. * versus the control.
