Protective Effects of Shen-Yuan-Dan, a Traditional Chinese Medicine, against Myocardial Ischemia/Reperfusion Injury In Vivo and In Vitro

Figure 1

Chromatographic profile of SYD pharmacological serum. A Shimadzu LC9A series HPLC system (SHIMADZU, Japan) with a SPD-6AV detector and a Kromasil 100-5C18 column (4.6 mm × 250 mm, 5 μm particle size) was used for HPLC analysis. The UV spectra were recorded in the range of 230–400 nm, and chromatographic peaks were measured at a wavelength of 280 nm. Mobile phase consisted of acetonitrile and 1% acetic acid (v/v). The flow rate was 1.0 mL/min and the column temperature was set at 25°C.