Protective Effects of Shen-Yuan-Dan, a Traditional Chinese Medicine, against Myocardial Ischemia/Reperfusion Injury In Vivo and In Vitro

Figure 4

Effects of SYD and LY294002 on cell apoptosis. (a) Representative images of Hoechst 33342 staining. (b) Quantitative analysis of apoptosis rate. The cells were exposed to 6 hours of normoxic culture (normal), 2 hours of hypoxia followed by 4 hours of reoxygenation (H/R), postconditioning with two concentrations of SYD or control serum (5% or 10% each). In some experiments, 5% SYD was complemented with LY294002 (5% SYD + LY294002). Data are expressed as mean ± SEM (𝑛=3), ^##𝑃<0.01 versus normal, ^∗∗𝑃<0.01 versus 5% control serum. ^ΔΔ𝑃<0.01 versus 10% SYD, and ^☆☆𝑃<0.01 versus 5% SYD. Scale bar represents 50 μm.