Zuo Jin Wan, a Traditional Chinese Herbal Formula, Reverses P-gp-Mediated MDR In Vitro and In Vivo
Figure 3
Effect of ZJW on apoptosis and cell cycle in HCT116/L-OHP cells. (a) Flow cytometry analysis of apoptosis with Annexin V-FITC/PI binding to HCT116/L-OHP cells. Viable cells (Annexin V−/PI−) are located in the lower left, apoptotic cells (Annexin V+/PI−) in the lower right, postapoptotic secondary necrotic cells (Annexin V+/PI+) in the upper right, and primary necrotic cells (Annexin V−/PI+) in the upper left quadrants, respectively. Numbers in each quadrant are percentages of cells. L-OHP group means HCT116/L-OHP cells exposure of L-OHP for 24 h; L-OHP + ZJW group means HCT116/L-OHP cells exposure of L-OHP and ZJW (50 μg/mL) for 12 h and HCT116/L-OHP cells without any treatment as the control. (b) Flow cytometry analysis of apoptosis with Annexin V-FITC/PI binding to HCT116/L-OHP cells after treatment with ZJW at 24 h, 36 h, and 48 h. (c) Flow cytometry analysis of cell cycle distribution in three groups which described as (a).
