Zuo Jin Wan, a Traditional Chinese Herbal Formula, Reverses P-gp-Mediated MDR In Vitro and In Vivo
Figure 5
Inhibition effect of ZJW on the ABCB1 gene and its encoded P-gp in vitro. (a) Western blotting assay was carried out to detect the level of P-gp, LRP, and MRP2 in HCT116 cells, HCT116/L-OHP cells, and HCT116/L-OHP cells treated with ZJW for at 25 μg/mL, 50 μg/mL, and 100 μg/mL for 48 h, respectively. Western blotting with an antibody to GAPDH was used to ensure equal loading of proteins in each lane. The bolts were photographed and quantitated for each sample; the data are from three independent experiments. (b) The activity of MDR1 promoter in HCT116/L-OHP cells treatment with ZJW at 24 h, 48 h, and 72 h was analyzed by dual-luciferase assay kit. The resulted data is firefly luciferase/renilla luciferase from different groups. Data are means ± standard deviation of values from triplicate experiments. , represents that ZJW treatment group is significantly different from control group at 24 h, 48 h, and 72 h.
