Zuo Jin Wan, a Traditional Chinese Herbal Formula, Reverses P-gp-Mediated MDR In Vitro and In Vivo
Figure 7
Inhibition effect of ZJW on the level of P-gp and the expression of MDR1 mRNA in vivo. (a) The xenograft tumor tissue in mice of all groups was subjected to immunohistology analysis using P-gp antibody; the results were magnified 200 in microscope; positive cells were brown staining; negative cells were blue staining. (b) The xenograft tumor tissues in mice of all groups were subjected to real-time qPCR to determine the mRNA expression of MDR1. Quantification of P-gp and MDR1 mRNA was performed by assigning a value of 1 to the group treated with H-ZJW (4110 mg/kg) + L-OHP (5 mg/kg). Statistical difference was analyzed by Student’s -test, versus control group; versus L-OHP alone group. This is a representative result of three repetitive experiments with similar results.
