Antitumor Effect of Periplocin in TRAIL-Resistant Human Hepatocellular Carcinoma Cells through Downregulation of IAPs
Figure 2
Periplocin and TRAIL treatments regulated cell apoptosis in HA22T/VGH cells. (a) HA22T/VGH cells were treated with periplocin alone or together with TRAIL for 24 h, and the morphology of cells was observed under microscope. (b) Cell apoptosis in HA22T/VGH cells regulated by periplocin and/or TRAIL was determined by FACS with Annexin-V-Fluorescein (-axis) and PI (-axis) double-staining system. (c) PI staining for both HA22T/VGH and Huh-7 cells after periplocin and/or TRAIL treatments. Apoptotic cells were quantified by FACS analysis (sub-G1 group).
