Higenamine Combined with [6]-Gingerol Suppresses Doxorubicin-Triggered Oxidative Stress and Apoptosis in Cardiomyocytes via Upregulation of PI3K/Akt Pathway
Figure 2
The components of Sini decoction protected NRCs from DOX-induced cell death. (a) Treatment schedules, cells were incubated with or without DOX (5 μM) for 12 h, followed by incubation in drug-free medium or higenamine (HG) or [6]-gingerol ([6]-GR) or glycyrrhizin (GC) for 12 h. (b) Effect of different concentrations of HG or [6]-GR or GC on cell viability in NRCs induced by DOX. (c) Effect of HG/[6]-GR combination, HG single, [6]-GR single, and DEX on cell viability in NRCs induced by DOX. DEX was incubated 1 h prior to DOX at a recommended dose of 10 times or 20 times that of DOX. Cell viability was determined by MTT assay. Results were expressed as percentages of control group. Data are shown as meanSD from three independent experiments. ** DOX group versus control group, #, ## versus DOX group. ▲ versus HG/[6]-GR combination group.
