Higenamine Combined with [6]-Gingerol Suppresses Doxorubicin-Triggered Oxidative Stress and Apoptosis in Cardiomyocytes via Upregulation of PI3K/Akt Pathway
Figure 7
Effect of HG/[6]-GR on the expression of Bcl-2 and Bax in NRCs with the ratio of Bax to Bcl-2 as quantitative analysis. (a) Measurement of protein expression by Western blot. NRCs were preincubated with 5 μM DOX for 12 h and treated with or without HG/[6]-GR for 12 h. Representative blots (upper) and quantitative analysis of Bcl-2 and Bax compared to -actin (lower) were given. (b) Measurement of mRNA by real-time PCR NRCs was similarly treated as indicated. After being treated as indicated, total RNA was subjected to quantitative real-time PCR analysis with specific primers for Bcl-2 and Bax. Experiments were repeated three times and data were shown as meanSD (relative to control group) after normalization to -actin. *, ** DOX group versus control group, ## DOX+HG/[6]-GR group versus DOX group. ▲, ▲▲versus HG/[6]-GR combination group.