Higenamine Combined with [6]-Gingerol Suppresses Doxorubicin-Triggered Oxidative Stress and Apoptosis in Cardiomyocytes via Upregulation of PI3K/Akt Pathway

Figure 8

HG/[6]-GR inhibited DOX-induced apoptosis via activation of Akt in NRCs. Effect of HG/[6]-GR on cell viability after addition of PI3-kinase inhibitor (LY294002). Effect of HG/[6]-GR on cytochrome c (a) and cleaved caspase-3 expression (b) after addition of LY294002. PI3K inhibitor LY294002 was added into the culture medium 60 min before cells lysis. Cell lysate was blotted with cytochrome c, cl-caspase-3, and procaspase-3. Scanning densitometry was used for semiquantitative analysis compared to 𝛽-actin level. Experiments were repeated three times and data were shown as mean±SD. **𝑃<0.01 DOX group versus control group, ^#𝑃<0.05 DOX+HG/[6]-GR group versus DOX group. ^▲𝑃<0.05 DOX+HG/[6]-GR+LY294002 group versus DOX+ HG/[6]-GR combination group.

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(b)