Higenamine Combined with [6]-Gingerol Suppresses Doxorubicin-Triggered Oxidative Stress and Apoptosis in Cardiomyocytes via Upregulation of PI3K/Akt Pathway

Figure 9

HG/[6]-GR inhibited DOX-induced apoptosis or cell death via activation of Akt in H9c2 cells. (a) Effect of HG/[6]-GR on cell viability after addition of PI3-kinase inhibitor (LY294002). ((b) and (c)) effect of HG/[6]-GR on MMP after addition of LY294002 (original magnification ×400, bar 100 μm). ((d) and (e)) flow histogram analysis of Annexin V staining. (A) control group, (B) DOX group, (C) DOX+HG/[6]-GR group, (D) HG/[6]-GR group; (E) HG/[6]-GR+LY294002 group, (F) DOX+HG/[6]-GR+LY294002 group. Cells were pretreated with 5 μM DOX for 12 h and then incubated with or without indicated concentrations of HG/[6]-GR for another 12 h. PI3K inhibitor LY294002 was added into the culture medium 60 min before cells harvest. Experiments were repeated at least three times and data were shown as mean±SD. *𝑃<0.05, **𝑃<0.01 DOX group versus control group, ^#𝑃<0.05, ^##𝑃<0.01 DOX+HG/[6]-GR group versus DOX group. ^▲𝑃<0.05, ^▲▲𝑃<0.01 DOX+HG/[6]-GR+LY294002 group versus DOX+ HG/[6]-GR combination group.