Curative Effects of Thiacremonone against Acetaminophen-Induced Acute Hepatic Failure via Inhibition of Proinflammatory Cytokines Production and Infiltration of Cytotoxic Immune Cells and Kupffer Cells
Figure 5
Reduction of the infiltration of immune cells and Kupffer cells into blood and liver tissues of thiacremonone pretreated mice. Blood ((a), (b), and (c)) and liver (d) tissues were separated at study termination. Flowcytometry analysis was performed using FACSCalibur flow cytometry. Representative data are shown. Data are mean ± SD of four experimental animals. (d) Photographs of the liver sections for Kupffer and NK cells after APAP in combination with vehicle or thiacremonone. Immunoreactivity with Kupffer and NK cells was expressed in the periportal (centrilobular) hepatocytes. APAP treatment exhibited the intense expression of Kupffer, NK, and Tc cells in the centrilobular sinusoids; however, pretreatment with thiacremonone caused mild staining for Kupffer and NK cells in the centrilobular sinusoids.
