Effects of Chinese Medicine Tong xinluo on Diabetic Nephropathy via Inhibiting TGF-β1-Induced Epithelial-to-Mesenchymal Transition
Figure 2
Time-course and dose-response of E-CA and α-SMA mRNA expressions in HKCs induced by TGF-β1. (a) HKCs were treated with five concentrations (5 ng/mL–25 ng/mL) of TGF-β1, and expression of E-CA mRNA was determined by RT-PCR with β-actin as an internal control. (b) Expression of α-SMA mRNA was determined by RT-PCR with β-actin as an internal control. (c) E-CA mRNA expression of cells exposed to 10 ng/mL TGF-β1 from 24 to 72 hours, normalized to β-actin. (d) α-SMA mRNA expression of cells exposed to 10 ng/mL TGF-β1 from 24 to 72 hours, normalized to β-actin. (e) Mean fluorescence activity of E-CA and α-SMA in normal HKCs and cells incubated with 10 ng/mL TGF-β1 for 48 hours. Photographs were analyzed by image-pro plus 6.0 software.(f) Representative immunofluorescence staining photographs of E-CA and α-SMA in normal HKCs and cells incubated with 10 ng/mL TGF-β1 for 48 hours. Images are shown at 40x and visualized by confocal microscope. (g) Representative photographs of phenotype change in HKCs induced by 10 ng/mL TGF-β1 compared with normal cells.
