Effects of Chinese Medicine Tong xinluo on Diabetic Nephropathy via Inhibiting TGF-β1-Induced Epithelial-to-Mesenchymal Transition
Figure 5
Effects of TXL on the expressions of Col IV and FN. (a) Col IV and FN mRNA expressions were detected by RT-PCR in vivo, normalized to β-actin. (b) Col IV and FN mRNA expressions were detected by RT-PCR in vitro, normalized to β-actin. (c) Representative photographs of Col IV and FN by immunofluorescence staining in renal tissues. Images are shown at 20x. (d) Mean fluorescence activity of Col IV and FN in renal tissues. Photographs were analyzed by image-pro plus 6.0 software. (e) Representative photographs of cells stained with primary antibody against Col IV and FN together with DAPI (blue). Images are shown at 40x. (f) Mean fluorescence activity of Col IV and FN detected by immunofluorescence staining. Photographs were analyzed by image-pro plus 6.0 software.
