Neuroprotective Effects of Cuscutae Semen in a Mouse Model of Parkinson’s Disease
Figure 4
CS protects against DA neuronal death due to MPTP-induced neurotoxicity. At 7 days after the final MPTP injection, the animals were sacrificed, and the brains were prepared for immunohistochemistry. Tyrosine hydroxylase (TH) immunostaining of the SNs of the mice treated with PBS (control), MPTP, and vehicle or MPTP and CS (50, 100, or 200 mg/kg). The representative images were displayed (a). The scale bars represent 500 μm. The number of TH-positive neurons in the SN measured (b). At 7 days after the final MPTP injection, the animals were sacrificed, and the brains were prepared for immunohistochemistry. The error bars represent the SEMs. , significantly different from the MPTP/PBS treated control group. , significantly different from the saline with PBS group (one-way ANOVA and Student-Newman-Keuls analyses). Dotted lines indicate the SN. The schematic brain sections are from the atlas (Paxinos and Watson, 1986). The square represents SN in the mouse brain. C: control; M: MPTP; CS50: CS50+MPTP; CS100: CS100+MPTP; CS200: CS200+MPTP.
