Effect of Rat Medicated Serum Containing You Gui Wan on Mouse Oocyte In Vitro Maturation and Subsequent Fertilization Competence

Figure 3

Effects of the rat medicated serum containing YGW on the percentage of in vitro fertilization and embryo cleavage. (a) shows a higher percentage of in vitro fertilization found in the rat medicated serum group when compared to the other three groups on day 1 after IVF (symbols *, , and # indicate comparison of rat medicated group with blank control group, normal rat serum group, and FSH group, resp.; ** indicates comparison of FSH group versus blank control group). (b) shows no significant difference in the embryo cleavage rate found among the groups on day 2 after IVF. (c) shows representative images of light micrographs of the fertilized oocytes and embryos on day 1 and day 2 after IVF among the blank control group, the normal rat serum group, the FSH group, and the rat medicated serum group. Scale bar is set to 100 μm.C, NS, FSH, and MS are abbreviations for blank control, normal rat serum, FSH, and rat medicated serum, respectively. IVM: in vitro maturation.

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