Effect of Rat Medicated Serum Containing You Gui Wan on Mouse Oocyte In Vitro Maturation and Subsequent Fertilization Competence
Table 1
List of the primers used in the qPCR analysis.
Genes
Primers
Amplified size
PKA
TACTTGGCCCCCGAGATTATC GCGAAGAAGGGTGGGTAACC
110 bp
CREB
TGCCCCTGGAGTTGTTATGG CTCTTGCTGCCTCCCTGTTC
110 bp
MAPK
ACCTCAGCAATGACCACATCTG CAGGAGGTTGGAAGGCTTGA
110 bp
MPF
CTCCAGGGAATTGTGTTTTGC AGGCCGAAATCAGCCAGTTT
110 bp
PKC
CGGCTGTACTTCGTCATGGA GAGATCTCGGCTGCGTAGAATAC
110 bp
PKG
TGCGAAGATTCTCATGCTCAA ACTGGCATTTGTGGAGTTTCCT
110 bp
β-ACTIN
TGGCATCCATGAAACTACATTCA TGCCTGGGTACATGGTGGTA
110 bp
PKA: protein kinase A; CREB: cAMP-response element binding protein; MAPK: mitogen-activated protein kinase; MPF: maturation-promoting factor; PKC: protein kinase C; PKG: protein kinase G.
