Immunostimulatory Activity of Protein Hydrolysate from Oviductus Ranae on Macrophage In Vitro

Figure 1

Effect of protein hydrolysate of Oviductus Ranae on cell viability. RAW 264.7 cells were pretreated with test substances (0, 10, 100, 500, 1000, and 2000 μg/mL) for 48 h and were used to test the cytotoxicity by MTT assay. OR, Oviductus Ranae; ORP, Oviductus Ranae protein; ORPH-pa, Oviductus Ranae protein hydrolysate prepared with papain; ORPH-tp, Oviductus Ranae protein hydrolysate prepared with trypsin; ORPH-pe, Oviductus Ranae protein hydrolysate prepared with pepsin; ORPH-np, Oviductus Ranae protein hydrolysate prepared with neutral protease; ORPH-ap, Oviductus Ranae protein hydrolysate prepared with alkaline protease. Control, untreated cells.