Immunostimulatory Activity of Protein Hydrolysate from Oviductus Ranae on Macrophage In Vitro

Figure 3

Effect of protein hydrolysate of Oviductus Ranae on NO production. (a) RAW 264.7 cells were pretreated with test substances (500 μg/mL) or LPS (1 μg/mL) for 48 h. The supernatant nitrite levels were determined using Griess reagent. Control, untreated. (b) RAW 264.7 cells were pretreated with ORPH-np (0, 10, 100, 500, and 1000 μg/mL) for 48 h, the supernatant nitrite levels were determined using Griess reagent. Control cells were pretreated with the medium that contained same levels of neutral protease with ORPH-np, the neutral protease was processed by the same procedure with ORPH. (c) RAW 264.7 cells were pretreated with ORPH-np (0, 10, 100, 500, and 1000 μg/mL) for 24, 48, and 72 h and the supernatant nitrite levels were determined using Griess reagent. Control, 0 μg/mL. Results were expressed as means ± S.D. of four separate experiments. Statistical significance test for comparison with untreated group was done by -test. ; .

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