Immunostimulatory Activity of Protein Hydrolysate from Oviductus Ranae on Macrophage In Vitro

Figure 4

Effect of ORPH on expression of iNOS in RAW 264.7 cells. (a) Cells were incubated with ORPH (500 μg/mL) or LPS (1 μg/mL) for 24 h. Total RNA was prepared and mRNA levels encoding iNOS was measured by RT-PCR. β-actin was used as an internal control. (b) Corresponding quantification data of iNOS mRNA expressional levels. (c) The protein level of iNOS in ORPH-stimulated RAW 264.7 cells was analyzed by Western blotting. (d) Corresponding quantification data of iNOS protein levels. ORPH, Oviductus Ranae protein hydrolysate prepared with neutral protease. Control, untreated. The results were stated in iNOS versus β-actin; data are expressed as mean ± SD (). Statistical significance test for comparison with untreated group was done by -test. ; .