Immunostimulatory Activity of Protein Hydrolysate from Oviductus Ranae on Macrophage In Vitro

Figure 5

Effects of ORPH on macrophage-related cytokine production in macrophages. RAW 264.7 cells (3 × 10⁵ cells/well) were cultured for 24 h in the presence of media with ORPH (0–1000 μg/mL) or LPS (1 μg/mL). The amounts of TNF-α (a), IL-1β (b), and IL-6 (c) released into the culture media were measured by immunoassays. ORPH, Oviductus Ranae protein hydrolysate prepared with neutral protease. Results were expressed as means ± S.D. of four separate experiments. Statistical significance test for comparison with untreated group was done by -test. ; .

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