Immunostimulatory Activity of Protein Hydrolysate from Oviductus Ranae on Macrophage In Vitro

Figure 6

Effect of the peptide with different molecular weights on the NO and TNF-α production of RAW 264.7 cells. (a) and (b) cells were pretreated with or without test substances (500 μg/mL), respectively. The supernatant nitrite levels and TNF-α were determined using Griess reagent and ELISA kit, respectively. Control, 0 μg/mL. (c) and (d) cells were cultured in the presence of media with the <3 kDa fraction (0–1000 μg/mL) or LPS (1 μg/mL). The amounts of NO and TNF-α in the supernatant were determined using Griess reagent and ELISA kit, respectively. ORPH, Oviductus Ranae protein hydrolysate prepared with neutral protease. Results were expressed as means ± S.D. of four separate experiments. Statistical significance test for comparison with untreated group was done by -test. ; .

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