Immunostimulatory Activity of Protein Hydrolysate from Oviductus Ranae on Macrophage In Vitro

Figure 7

Effect of ORPH on nuclear NF-κB. (a) and cytoplasmic IκB-α (b) expression level by RAW 264.7 cells. Cells were cultured for 24 h in the presence of media with ORPH (0–500 μg/mL) or LPS (1 μg/mL). Western blotting was performed to detect the protein level of nuclear NF-κB and cytoplasmic IκB-α. The levels of lamin B were measured as internal loading controls. The results were analyzed by iPP 6.0 software and stated in NF-jB versus Lamin B, data are expressed as mean ± S.D. (). Statistical significance test for comparison with untreated group was done by -test. ; .

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(b)