Effect of Gloriosa superba and Catharanthus roseus Extracts on IFN-γ-Induced Keratin 17 Expression in HaCaT Human Keratinocytes

Figure 4

K17 expression as determined using Western blot was low in untreated group, but its overexpression was found to be induced by 1 ng/mL of IFN-γ (pretreated). IFN-γ-induced K17 expression was decreased when treated with G. superba extract (working concentrations of 0.1, 1, 10, and 100 μg/mL) and C. roseus extract (working concentrations of 100, 250, 500, and 1000 μg/mL) in the dose-dependent manner as shown in representative Western blot ((a) and (b)) and normalized values of K17 against GAPDH ((c) and (d)). The final concentrations of G. superba extract were 0.01, 0.1, 1, and 10 μg/mL, while the final concentrations of C. roseus extract were 10, 25, 50, and 100 μg/mL. The mean ± SD values of normalized K17 expression were obtained from 3 independent experiments. The effect of both extracts on K17 expression in HaCaT was also detected by immunofluorescence staining. K17 expression was in untreated and IFN-γ-treated groups (e). The effect of G. superba extract at 4 concentrations (final tested concentrations = 0.01, 0.1, 1, and 10 ug/mL, resp.) on K17 expression (f). The effect of C. roseus extract at 4 concentrations (final tested concentrations = 10, 25, 50, and 100 ug/mL, resp.) on K17 expression (g).

(a) G. superba extract

(b) C. roseus extract

(c) G. superba extract

(d) C. roseus extract

(e)

(f) G. superba extract

(g) C. roseus extract