Effect of Gloriosa superba and Catharanthus roseus Extracts on IFN-γ-Induced Keratin 17 Expression in HaCaT Human Keratinocytes

Figure 5

The expression of p-STAT3 was high in both untreated and IFN-γ groups. Its expression was significantly decreased when treated with G. superba extract (working concentrations of 0.1, 1, 10, and 100 μg/mL) and was found to decline when treated with C. roseus extract (working concentrations of 100, 250, 500, and 1000 μg/mL) as shown in representative Western blot ((a) and (b)) and normalized values of p-STAT3 against GAPDH ((c) and (d)). The final concentrations of G. superba extract were 0.01, 0.1, 1, and 10 μg/mL, whereas the final concentrations of C. roseus extract were 10, 25, 50, and 100 μg/mL. The mean ± SD values of normalized p-STAT3 expression were obtained from 3 independent experiments.

(a) G. superba extract

(b) C. roseus extract

(c) G. superba extract

(d) C. roseus extract