Antitumor Activity of Chinese Propolis in Human Breast Cancer MCF-7 and MDA-MB-231 Cells

Figure 3

EECP induced apoptosis in MCF-7 and MDA-MB-231 cells. (a) Morphological changes of nuclei in MCF-7 cells by staining with acridine orange and Hoechst 33258 at 48 h (×200). (b) Morphological changes of nuclei in MDA-MB-231 cells by staining with acridine orange and Hoechst 33258 at 48 h (×200). (c) The levels of procaspase 3 (35KD) were detected by western blot at 24 h. (d) The hemiquantification of procaspase 3 levels in MCF-7 and MDA-MB-231 cells (^∗𝑃<0.05, ^∗∗𝑃<0.01 versus control, 𝑛=3).

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