Ganoderma lucidum Polysaccharides Reduce Lipopolysaccharide-Induced Interleukin-1β Expression in Cultured Smooth Muscle Cells and in Thoracic Aortas in Mice
Figure 4
EORPs reduce IL-1β expression in LPS-treated mice at the acute inflammatory phase (2 days). (a) Immunohistochemical staining for CD31 (an endothelial cell marker, left panels), IL-1β (middle panels), and α-actin (a smooth muscle cell marker, right panels) antibodies in serial sections of thoracic aortas. The lumen is uppermost in all sections. The arrowhead and the arrow indicate IL-1β-positive overlapping with endothelial cells and smooth muscle cells specific staining, respectively. Bar = 50 μm. (b) IL-1β concentration in the plasma was detected by ELISA. The data are the mean ± SEM (). * as compared to the saline-treated mice (control); † as compared to the LPS-treated mice. (c) Western blot analysis of IL-1β expression in thoracic aortas. β-actin was used as the loading control.
