Ganoderma lucidum Polysaccharides Reduce Lipopolysaccharide-Induced Interleukin-1β Expression in Cultured Smooth Muscle Cells and in Thoracic Aortas in Mice
Figure 5
EORPs reduce IL-1β expression in LPS-treated mice at the chronic inflammatory phase (2 weeks). (a) Immunohistochemical staining for CD31 (left panels), IL-1β (middle panels), and α-actin (right panels) antibodies in serial sections on thoracic aortas. The lumen is uppermost in all sections. The arrowhead and the arrow indicate IL-1β-positive cells overlapping with endothelial cells and smooth muscle cells specific staining, respectively. Bar = 50 μm. (b) IL-1β concentration in the plasma was detected by ELISA. The data are the mean ± SEM (). * as compared to the saline-treated mice (control); † as compared to the LPS-treated mice. (c) Western blot analysis of IL-1β protein level in thoracic aortas. The expression ratio (IL-1β/β-actin) was decreased in the EORP (LPS/EORP) group when compared to the LPS-treated group. β-actin was used as the loading control.
