Ganoderma lucidum Polysaccharides Reduce Lipopolysaccharide-Induced Interleukin-1β Expression in Cultured Smooth Muscle Cells and in Thoracic Aortas in Mice
Figure 6
EORP regulating the LPS-induced IL-1β expression was mediated through the TLR4 receptor. (a) Immunofluorescent staining for TLR4. HASMCs were left untreated or incubated for 24 h with or without 10 μg/mL EORP and then incubated with the 10 μg/mL LPS for another 24 h. TLR4 expression was indicated by green fluorescence (FITC) and nuclei by blue fluorescence (DAPI). The untreated HASMCs were used as the control cells. Bar = 100 μm. (b) Immunohistochemical staining for IL-1β expression in thoracic aortas of TLR4−/− mice. The lumen is uppermost in all sections. Bar = 50 μm. (c) IL-1β concentration in plasma was detected by ELISA. The data are the mean ± SEM (). (d) Western blot analysis of IL-1β in thoracic aortas of TLR4−/− mice. The saline-treated TLR4−/− mice were used as the control group in (b), (c), and (d). β-actin was used as the loading control. (e) Western blot analysis of TLR4 expression in thoracic aortas of C57BL6J (con) and TLR4−/− mice. GAPDH was used as the loading control. F: CAGACCATGATCACACAGGG R: TGGAAAGATGGGCCTGTTAG.
