KCHO-1, a Novel Antineuroinflammatory Agent, Inhibits Lipopolysaccharide-Induced Neuroinflammatory Responses through Nrf2-Mediated Heme Oxygenase-1 Expression in Mouse BV2 Microglia Cells

Figure 5

The effects of KCHO-1 on HO-1 mRNA (a) and protein (b) expression and the nuclear translocation of Nrf2 (c, d) in BV2 microglia. Cells were incubated for 12 h with the indicated concentrations of KCHO-1 (a, b) or treated with 200 μg/mL of KCHO-1 for 0.5, 1, and 1.5 h (c, d). RNA quantification and western blot analysis for mHO-1 and mGAPDH expression were performed, and the nuclei were fractionated from the cytosol using PER-Mammalian Protein Extraction Buffer, as described in the Materials and Methods. The blots are representative of 3 independent experiments. compared with the control group.

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