KCHO-1, a Novel Antineuroinflammatory Agent, Inhibits Lipopolysaccharide-Induced Neuroinflammatory Responses through Nrf2-Mediated Heme Oxygenase-1 Expression in Mouse BV2 Microglia Cells

Figure 6

HO-1 mediates the suppressive effect of KCHO-1 on LPS-stimulated proinflammatory mediator production and NF-κB DNA binding. BV2 microglia were pretreated for 12 h with KCHO-1 (200 μg/mL), in the presence or absence of SnPP (50 μM), and stimulated for 18 h (a, b, c, d, e) or 1 h (f) with LPS (1 μg/mL). The concentrations of PGE₂ (a), NO (b), TNF-α (c), IL-1β (d), and IL-6 (e) were determined using ELISA kits, as described in the Materials and Methods. A commercially available NF-κB enzyme-linked immunosorbent assay (ELISA) kit (Active Motif) was used to test nuclear extracts and determine the degree of NF-κB binding (f). Cells were pretreated with SnPP for 3 h in this experiment. The data represent the mean values from 3 experiments ± SD. .

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