GBE50 Attenuates Inflammatory Response by Inhibiting the p38 MAPK and NF-κB Pathways in LPS-Stimulated Microglial Cells
Figure 4
The effect of GBE50 on NF-κB p65 nuclear translocation in LPS-activated BV2 cells. (a) There is almost no expression of NF-κB p65 in the nucleus of the control group. (b) In the LPS group, there is almost complete translocation of NF-κB p65 from the cytoplasm to the nucleus. (c)–(g) NF-κBp65 nuclear translocation was gradually inhibited at GBE50 concentrations of 1, 10, and 100 μg/mL. (h) EGB761 also had the obvious inhibitory effects on NF-κB p65 nuclear translocation. Confocal microscopy images of BV2 cells were stained with an antibody against NF-κB p65 (green) and counterstained with DAPI (blue) to label nuclei. Confocal images were captured through the center of control and WAVE2-KD acini immunostained for E-cadherin (green) and counterstained with Alexa-568 phalloidin (red) to label actin filaments and DAPI (blue) to label nuclei. White arrowheads showed that NF-κB p65 mainly localized in the cytoplasm and red arrowheads indicated the nuclear translocation of NF-κB p65. Scale bar = 10 μm.
