Role of JNK Activation and Mitochondrial Bax Translocation in Allicin-Induced Apoptosis in Human Ovarian Cancer SKOV3 Cells
Figure 4
Western blot analysis showing cytochrome and Bax levels in response to allicin. (a) SKOV3 cells were treated with 25 μg/mL of allicin for 12 h. Subsequently, cytosolic and mitochondrial fractions were prepared and western blot analysis was carried out (20 μg of protein) as described in Materials and Methods. (b) Pretreatment with or without the JNK inhibitor SP600125 for 30 min, followed by treatment with allicin for 12 h to analyze Bax and cytochrome . Data are representative of three independent experiments showing a similar pattern of expression. β-Actin and Hps60 were used as internal control.
