Ethanol Extract of Alismatis rhizome Inhibits Adipocyte Differentiation of OP9 Cells
Figure 2
Effects of AOE on lipid droplet formation and PPAR expression in OP9 cells. OP9 cells were treated with adipogenic inducers to trigger differentiation into adipocytes. AOE 20 (40 μg/mL) was then added during the early stage (0–2 days), late stage (3–5 days), or the entire period of differentiation (0–5 days). (a) After 5 days of differentiation, immunohistochemical staining of OP9 cells was carried out using a specific antibody to visualize PPAR (red), BODIPY 493/503 for lipid droplets (green), and DAPI to visualize nuclei (blue). (b) Total cellular lipid droplet content was determined by averaging cytosolic BODIPY intensities of individual cells. (c) PPAR levels were determined by averaging the intensities of nuclear antibody staining in individual cells. Approximately 5,000 cells were analyzed for lipid droplet content and PPAR levels. Data are expressed mean SD values of at least 3 independent experiments. compared to the D group. ND: no differentiation, D: differentiation.
