Ethanol Extract of Alismatis rhizome Inhibits Adipocyte Differentiation of OP9 Cells
Figure 3
Effects of AOE on expression of PPAR and PPAR-target genes. OP9 cells were treated with adipogenic inducers to trigger differentiation into adipocytes. AOE (40 μg/mL) was added during the early stage (0–2 days), late stage (3–5 days), or for the entire differentiation period (0–5 days). After 5 days of differentiation, real-time PCR was carried out using specific primers for PPAR, C/EBP, aP2, FAS, HSL, and LPL, as described in Table 1. The genes transcript levels are expressed as ratios relative GAPDH expression, with the level in ND to 1. Data are expressed as mean SD values of at least 3 independent experiments. ND: no differentiation, D: differentiation.
