Ethanol Extract of Alismatis rhizome Inhibits Adipocyte Differentiation of OP9 Cells
Figure 5
Effects of AOE on cell proliferation and autophagy-related protein expression in OP9 cells. OP9 cells pretreated with 40 μg/mL AOE were then cultured with adipogenic inducers for the designated times. After harvesting, the lysates were subjected to western blot analysis for cyclins A, D1, and D2 (a) and ERK, p-ERK, Akt, and p-Akt (b) and beclin1, Atg7, Atg12, and LC3B I and II (c). ND: no differentiation, D: differentiation, D1: differentiation day 1.
