In Vivo and In Vitro Antitumor Effects of Platycodin D, a Saponin Purified from Platycodi Radix on the H520 Lung Cancer Cell

Table 5

Changes on the spleen, submandibular lymph node, and periovarian fat fad histomorphometry in H520 tumor cell xenograft mice.

Organs	Groups
	Intact control	TB control	Gemcitabine	Platycodin D
				200 mg/kg	100 mg/kg	50 mg/kg
Spleen
Total thickness (mm)	1.75 ± 0.24	1.08 ± 0.17^a	1.02 ± 0.18^a	1.50 ± 0.14^ab	1.39 ± 0.14^ab	1.29 ± 0.15^ac
White pulp numbers (/mm^2)	15.75 ± 2.92	5.50 ± 1.41^a	5.13 ± 1.25^a	13.13 ± 1.46^ab	11.00 ± 2.14^ab	8.13 ± 1.13^ab
White pulp diameters (m)	489.11 ± 110.18	213.69 ± 30.71^d	216.96 ± 40.09^d	361.61 ± 50.97^ef	309.88 ± 73.63^df	281.75 ± 64.50^de
Submandibular lymph node
Total thickness (m)	993.00 ± 147.40	529.98 ± 72.52^a	557.65 ± 74.26^a	804.88 ± 112.71^ab	753.23 ± 109.45^ab	701.97 ± 97.42^ab
Cortex follicle numbers (/mm^2)	17.13 ± 1.55	6.63 ± 1.06^a	6.25 ± 1.83^a	13.50 ± 2.88^ab	11.88 ± 2.42^ab	9.88 ± 1.96^ab
Cortex thickness (m)	502.88 ± 101.13	245.84 ± 40.07^d	239.39 ± 39.83^d	408.00 ± 52.31^ef	360.78 ± 50.06^df	299.68 ± 28.60^dg
Periovarian fat pad
Total thickness (mm)	1.84 ± 0.25	0.42 ± 0.06^d	0.33 ± 0.07^dg	1.24 ± 0.23^df	0.97 ± 0.11^df	0.75 ± 0.19^df
White adipocyte diameters (m)	50.23 ± 5.93	16.10 ± 2.51^d	10.70 ± 2.20^dg	35.59 ± 5.95^df	28.07 ± 4.03^df	23.16 ± 4.15^df

Values are expressed mean ± S.D. of eight mice.
TB = tumor bearing.
Gemcitabine was intraperitoneally administered at 160 mg/kg, 7-day intervals.
Platycodin D was orally administered, once a day.
^a as compared with intact control by LSD test.
^b and ^c as compared with TB control by LSD test.
^d and ^e as compared with intact control by MW test.
^f and ^g as compared with TB control by MW test.