In Vivo and In Vitro Antitumor Effects of Platycodin D, a Saponin Purified from Platycodi Radix on the H520 Lung Cancer Cell
Table 5
Changes on the spleen, submandibular lymph node, and periovarian fat fad histomorphometry in H520 tumor cell xenograft mice.
Organs
Groups
Intact control
TB control
Gemcitabine
Platycodin D
200 mg/kg
100 mg/kg
50 mg/kg
Spleen
Total thickness (mm)
1.75 ± 0.24
1.08 ± 0.17a
1.02 ± 0.18a
1.50 ± 0.14ab
1.39 ± 0.14ab
1.29 ± 0.15ac
White pulp numbers (/mm2)
15.75 ± 2.92
5.50 ± 1.41a
5.13 ± 1.25a
13.13 ± 1.46ab
11.00 ± 2.14ab
8.13 ± 1.13ab
White pulp diameters (m)
489.11 ± 110.18
213.69 ± 30.71d
216.96 ± 40.09d
361.61 ± 50.97ef
309.88 ± 73.63df
281.75 ± 64.50de
Submandibular lymph node
Total thickness (m)
993.00 ± 147.40
529.98 ± 72.52a
557.65 ± 74.26a
804.88 ± 112.71ab
753.23 ± 109.45ab
701.97 ± 97.42ab
Cortex follicle numbers (/mm2)
17.13 ± 1.55
6.63 ± 1.06a
6.25 ± 1.83a
13.50 ± 2.88ab
11.88 ± 2.42ab
9.88 ± 1.96ab
Cortex thickness (m)
502.88 ± 101.13
245.84 ± 40.07d
239.39 ± 39.83d
408.00 ± 52.31ef
360.78 ± 50.06df
299.68 ± 28.60dg
Periovarian fat pad
Total thickness (mm)
1.84 ± 0.25
0.42 ± 0.06d
0.33 ± 0.07dg
1.24 ± 0.23df
0.97 ± 0.11df
0.75 ± 0.19df
White adipocyte diameters (m)
50.23 ± 5.93
16.10 ± 2.51d
10.70 ± 2.20dg
35.59 ± 5.95df
28.07 ± 4.03df
23.16 ± 4.15df
Values are expressed mean ± S.D. of eight mice. TB = tumor bearing. Gemcitabine was intraperitoneally administered at 160 mg/kg, 7-day intervals. Platycodin D was orally administered, once a day.
a as compared with intact control by LSD test.
b and c as compared with TB control by LSD test.
d and e as compared with intact control by MW test.
f and g as compared with TB control by MW test.