495840.fig.003a
(a)
495840.fig.003b
(b)
495840.fig.003c
(c)
Figure 3: 4-Hydroxymephenytoin induced insulin secretion in RIN-5F cells at a nontoxic concentration. (a) Subnetwork targeted by 4-Hydroxymephenytoin. (b) Effects of nateglinide, berberine, and 4-Hydroxymephenytoin on glucose-stimulated insulin secretion from RIN-5F islet cells. Cells were incubated with various concentrations of nateglinide, berberine, and 4-Hydroxymephenytoin for 2 h to induce insulin secretion. Glucose with different concentrations was used as the control for basal and hyperglycemic conditions. Values are means ± SD of five replicate experiments in each group. and are compared with the control group of 20 mM glucose. (c) Effects of nateglinide, berberine, and 4-Hydroxymephenytoin on cytotoxicity in RIN-5F islet cells. Cells were incubated with various concentrations of nateglinide, berberine, and 4-Hydroxymephenytoin, respectively for 24 h in the presence of 20 mM glucose. Cell viability was measured using MTT assay. Values are means ± SD of seven replicate experiments in each group.