495840.fig.004a
(a)
495840.fig.004b
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495840.fig.004c
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Figure 4: 4-Hydroxymephenytoin induced insulin-induced glucose consumption in 3T3-L1 adipocytes. (a) General view of differentiation of 3T3-L1 preadipocytes into adipocytes as seen in inverted phase contrast microscope (A–D). Large lipid droplets are the main characteristics of the cytoplasm of the cells (C). Intracellular lipid content was measured by oil red O staining. (b) Effects of pioglitazone, berberine, and 4-Hydroxymephenytoin on glucose consumption in 3T3-L1 adipocytes. Differentiated 3T3-L1 adipocytes in 96-well plates were preincubated with DMEM containing 0.2% BSA for 12 h and then incubated with various concentrations (0.1, 0.3, and 3 μM) of pioglitazone, berberine, and 4-Hydroxymephenytoin for 24 h. Glucose consumption amount was obtained from the difference in glucose concentrations between initial and final states for the indicated time from the culture medium. Values are means ± SD of five replicate experiments in each group. and are compared with model group. (c) Effects of pioglitazone, berberine, and 4-Hydroxymephenytoin on cytotoxicity in 3T3-L1 adipocytes. Cells were incubated with various concentrations of pioglitazone, berberine, and 4-Hydroxymephenytoin for 24 h. Cell viability was measured using MTT assay. Values are means ± SD of seven replicate experiments in each group.